dGH can be employed at multiple time points to monitor chromosomal structure throughout the lifetime of the editing process.
Pre-edit: dGH provides a measurement of pre-existing structural variation which is unique to each model, person and cell type. In addition to establishing a genomic baseline, dGH can be used for process optimization. dGH can use the on-and off-target rates of structural variation to monitor the effects of differing process variables including engineered-nuclease variants, guide strand choice and delivery method, and direct comparisons within the cell lines of interest.
Post-edit: dGH can measure structural rearrangements at multiple time points immediately following editing. In addition, dGH can be used to track the persistence of measured variants over time.
From establishing a baseline structural variation rate, to measuring editing-associated changes, to tracking genomic integrity after treatment, dGH provides a comprehensive structural genomic profiling technique which helps to ensure that any genomic rearrangements resulting from editing are accounted for.