TRACK THE FATE OF A GENE OF INTEREST
The KromaTiD dGH gene tracking platform combines a chromatid paint (or smaller subset of probes) labeled with one fluorophore, and gene-specific probes labeled with a contrasting fluorophore in a single multiplex assay. The different fluorescent tags enable a gene of interest to be ‘tracked’ in the context of surrounding genomic sequences. Based on Directional Genomic Hybridization™ (dGH™) technology, gene tracking probes hybridize to just one chromatid, making it easy to visualize structural rearrangements -including translocations, deletions and previously difficult to detect inversions-in the target gene. Since gene tracking assays generate data for single cells, not pools of cells, they are ideal for mixed cell population studies. Benefits include:
- Detection of Structural Rearrangements in Cell Populations: Gene tracking assays are capable of detecting structural rearrangements within a gene; they are ideal both for confirming known rearrangements and for discovering new ones. In an unstable population of cells, such as within a tumor, gene tracking assays can easily characterize different rearrangements.
- Customizable for Specific Research Needs: Gene tracking assays are available for most genes in the human genome and any previously-sequenced mammalian genome. Assay components and protocols are completely customized to fit client research needs.
- Rapid Development and Delivery: Custom dGH gene tracking assays are available in 50-assay kits. Unlike other chromosome imaging technology made-to-order assays that take months to develop, KromaTiD can generate a personalized gene tracking assay within a matter of weeks.