Concept Intro and Feedback:

dGH 5 Color, Whole-Genome Evaluation

KromaTiD is launching a single cell assay service measuring structural variants and genomic integrity across the whole genome using our proprietary high-density chromatid paints. This assay will comprehensively measure chromosomal rearrangements for each cell analyzed with <10Kb limit of detection. Dedicated to measurement of consistent structural variants and launching  in July 2020, KromaTiD’s 5CWG service is the first of a suite of single cell, whole genome karyographic assays. Future launches will include assays measuring genomic structural instability and variability and also banded dGH tools and targeted probes to help further localize breakpoints and structural variants.

Using the reference genome as a baseline, our new 5CWG service will provide single-cell data that will enable researchers to screen the whole genome in an entirely de novo fashion. These single cell results are then compared across a large number of cells to provide a complete structural profile of the sample, regardless of the degree of structural heterogeneity. The 5CWG assay will generate the most comprehensive hypothesis-free perspective of genomic structural variation to date by directly imaging the entire genome of individual cells. Chromosome ID is assigned through a combination of color, size and centromeric position.

The 5CWG product is a standard assay designed to discover and quantify consistent structural variants (e.g. involving your edit site), structural mosaiscism, structural variants that drive rare diseases or sub-clonal populations in your cell lines. At launch, KromaTiD will provide these measurements for blood derived cells, cell lines, IPSC and other stem cells. Our standard service will measure structural variants in 50 cells, provide a standard report of results, trends and example images. Typical turnaround time will be 2 weeks.

Structural Variants Measured

  • Exchange Events
    • Reciprocal & Non-reciprocal Translocations
    • Balanced & Unbalanced Translocations​
    • Allelic Translocations
    • Large InDels
  • Orientation Events
    • Inversions
    • Recombinations
    • Sister Chromatid Exchanges
  • Chromosomal Gain/Loss and Rearrangements
    • Sister Chromatid Fusions
    • Gross Insertions and Deletions (Gain/Loss)
    • Dicentrics and Acentrics
    • Fragmentation/Chromothrypsis
    • Polyploidy
    • Aneuploidy
    • Monosomy
    • Polysomy
5CWG With Rearrangements