Replicated Chromosome Metaphase Configuration. Pre-Replication G1 Chromosome. *Inverted Segment. 1) Selective Daughter Strand Degradation 2) Single-Strand dGH Probes 3) Imaging. Visual Orientation Data. When used on metaphase chromosomes, dGH is the only imaging technology capable of providing sequence, location and orientation information in a single assay. The KromaTiD platform enables orders of magnitude higher-resolution inversion detection than any competing technique. Chromosome and Chromatid Assay Format. Using the same probes in different test conditions, KromaTiD assays are capable of targeting entire chromosomes (double-stranded applications like FISH) or individual chromatids (single-stranded applications) in interphase or metaphase cells. Quantitative Mutation Size and Location. Because dGH assays are based on a defined library of sequenced and calibrated genomic probes, they can precisely locate and quantitatively size rearrangements. Unique Specificity. Probes are designed to target unique genomic sequences, so KromaTiD assays require no blocking DNA (COT), exhibit no non-specific background, and demonstrate improved hybridization performance. Single Cell Analysis. dGH assays generate imaging data for single cells, not pools of cells, so are ideal for determining mutation heterogeneity within mixed cell populations while simultaneously identifying recurrent rearrangements. The Broadest Assay Range. In a single assay, KromaTiD products detect the broadest possible spectrum of chromosome rearrangements, including those assayable by standard FISH technologies (e.g. translocations between chromosomes) as well as intra-chromosomal rearrangements such as cryptic inversions.