THE PLATFORM

Orientation Detection: For the discovery or detection of inversions, proprietary dGH probes are used with an innovative chromosome preparation in a technique that is capable of determining orientation information from imaging data.1

Imaging System Compatibility: KromaTiD assays can be run on existing chromosome imaging equipment, and therefore require minimal training and no additional capital investment.

Optimal Performance: When used as Pinpoint FISH reagents with standard FISH methodologies and equipment, KromaTiD’s dGH probes maximize assay performance by improving kinetics, reducing background and eliminating the need for blocking DNA (COT).

1Ray FA, Zimmerman E, Robinson B, Cornforth MN, Bedford JS, Goodwin EH, Bailey SM (2013) Directional genomic hybridization for chromosomal inversion discovery and detection.  Chromosome Res 21(2):165-174

TRANSFORMATIONAL CHROMOSOME IMAGING TOOLS

KromaTiD’s directional Genomic Hybridization™ (dGH) technology combines bioinformatics driven design of unique single-stranded synthetic probes with strand-specific hybridization strategies, and is the only genomics approach capable of detecting DNA sequence, location and orientation in a single test. Assay design with dGH™ provides extremely strong signal strength, eliminates background, and detects small chromosomal rearrangements with high resolution.

FLEXIBLE, HIGH-PERFORMANCE CHROMOSOME ASSAYS

The foundation of the dGH platform is a library of proprietary short, synthetic, single-stranded DNA probes. Together with unique and optimized assay methods, the dGH platform provides researchers with:

Visual Orientation Data:

When used on metaphase chromosomes, dGH is the only imaging technology capable of providing sequence, location and orientation information in a single assay.

The KromaTiD platform enables orders of magnitude higher-resolution inversion detection than any competing technique.

Chromosome and Chromatid Assay Formats:

Using the same probes in different test conditions, KromaTiD assays are capable of targeting entire chromosomes (double-stranded applications like FISH) or individual chromatids (single-stranded applications) in interphase or metaphase cells.

Quantitative Mutation Size and Location:

Because dGH assays are based on a defined library of sequenced and calibrated genomic probes, they can precisely locate and quantitatively size rearrangements.

Unique Specificity:

Probes are designed to target unique genomic sequences, so KromaTiD assays require no blocking DNA (COT), exhibit no non-specific background, and demonstrate improved hybridization performance.

Single Cell Analysis:

dGH assays generate imaging data for single cells, not pools of cells, so are ideal for determining mutation heterogeneity within mixed cell populations while simultaneously identifying recurrent rearrangements.

The Broadest Assay Range:

In a single assay, KromaTiD products detect the broadest possible spectrum of chromosome rearrangements, including those assayable by standard FISH technologies (e.g. translocations between chromosomes) as well as intra-chromosomal rearrangements such as cryptic inversions.

ASSAYS DESIGNED TO FIT YOUR RESEARCH NEEDS

Taking advantage of cutting-edge genomics and bioinformatics strategies, Directional Genomic Hybridization can be efficiently deployed in projects ranging from one to thousands of samples. With the ability to resolve very small inversions and translocations, dGH technology can be employed in both directed and de novo discovery studies, quickly providing data on new or variable rearrangements for your patient screening or target validation needs.  If a complete genetic profile is required, pair dGH with NGS on the same samples to build a comprehensive mutation profile.

For inversions or translocations with known breakpoints, targeted dGH assays are an efficient method of screening large populations or libraries, and can be multiplexed and automated to provide data on multiple aberrations in the same test.

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